Complement activation and pulmonary leukotasis during nylon fiber filtration leukapheresis

Nylon Fiber Leukapheresis : Associated Complement

The possible role of complement activation in the development of transient granulocytopenia during nylon fiber filtration leukapheresis ( FL) was studied in five normal donors. No changes in hemolytic complement (CH50) titers were observed in the donors’ circulating plasma at any point in the procedure. However, comparison of preand postfilter blood samples drawn 1 mm after donor blood passed t...

Detection, pathogenesis, and prevention of damage to human granulocytes caused by interaction with nylon wool fiber. Implications for filtration leukapheresis.

Granulocytes collected by reversible adhesion to nylon wool fiber (NWF) function relatively well in standard in vitro tests; however, they have an abnormally shortened survival time in the circulation. Assuming that this rapid disappearance represents clearance and that recognition by phagocytes is important for such clearance, we used an autologous in vitro cell:cell recognition assay to deter...

Mobilization and exocytosis of specific (secondary) granules by human neutrophils during adherence to nylon wood in filtration leukapheresis (FL).

We studied the interaction of human neutrophils with nylon wool in vitro and during filtration Ieukapheresis (FL) to better understand cellular events detrimental to neutrophils that occur during FL. When neutrophils purified from blood by gradient centrifugation and sedimentation were incubated with nylon wool in vitro, release of lysozyme from the cells occurred rapidly and to a degree that w...

Reversal of granulocyte adherence to nylon fibers using local anesthetic agents: possible application to filtration leukapheresis.

The effects of the cationic anesthetic agents tetracaine and lidocaine on granulocyte function, morphology, and adherence to nylon fibers were studied in an attempt to improve current methods of granulocyte collection by filtration leukapheresis (FL). When dissolved in acid-citrate-dextrose (ACD) plasma, these drugs significantly increased granulocyte elution from the fibers in a dose-related f...

Filtration leukapheresis (FL) and granulocytes.